19 lines
1.4 KiB
Plaintext
19 lines
1.4 KiB
Plaintext
TY - JOUR
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AU - Al Ouahabi, Abdelaziz
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AU - Amalian, Jean-Arthur
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AU - Charles, Laurence
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AU - Lutz, Jean-François
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PY - 2017
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DA - 2017/10/17
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TI - Mass spectrometry sequencing of long digital polymers facilitated by programmed inter-byte fragmentation
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JO - Nature Communications
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SP - 967
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VL - 8
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IS - 1
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AB - In the context of data storage miniaturization, it was recently shown that digital information can be stored in the monomer sequences of non-natural macromolecules. However, the sequencing of such digital polymers is currently limited to short chains. Here, we report that intact multi-byte digital polymers can be sequenced in a moderate resolution mass spectrometer and that full sequence coverage can be attained without requiring pre-analysis digestion or the help of sequence databases. In order to do so, the polymers are designed to undergo controlled fragmentations in collision-induced dissociation conditions. Each byte of the sequence is labeled by an identification tag and a weak alkoxyamine group is placed between 2 bytes. As a consequence of this design, the NO-C bonds break first upon collisional activation, thus leading to a pattern of mass tag-shifted intact bytes. Afterwards, each byte is individually sequenced in pseudo-MS3 conditions and the whole sequence is found.
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SN - 2041-1723
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UR - https://doi.org/10.1038/s41467-017-01104-3
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DO - 10.1038/s41467-017-01104-3
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ID - Al Ouahabi2017
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ER -
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