Laptop backup week 10? (for safety reasons)

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Aidan Sharpe
2025-03-29 23:46:27 -04:00
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TY - JOUR
AU - Al Ouahabi, Abdelaziz
AU - Amalian, Jean-Arthur
AU - Charles, Laurence
AU - Lutz, Jean-François
PY - 2017
DA - 2017/10/17
TI - Mass spectrometry sequencing of long digital polymers facilitated by programmed inter-byte fragmentation
JO - Nature Communications
SP - 967
VL - 8
IS - 1
AB - In the context of data storage miniaturization, it was recently shown that digital information can be stored in the monomer sequences of non-natural macromolecules. However, the sequencing of such digital polymers is currently limited to short chains. Here, we report that intact multi-byte digital polymers can be sequenced in a moderate resolution mass spectrometer and that full sequence coverage can be attained without requiring pre-analysis digestion or the help of sequence databases. In order to do so, the polymers are designed to undergo controlled fragmentations in collision-induced dissociation conditions. Each byte of the sequence is labeled by an identification tag and a weak alkoxyamine group is placed between 2 bytes. As a consequence of this design, the NO-C bonds break first upon collisional activation, thus leading to a pattern of mass tag-shifted intact bytes. Afterwards, each byte is individually sequenced in pseudo-MS3 conditions and the whole sequence is found.
SN - 2041-1723
UR - https://doi.org/10.1038/s41467-017-01104-3
DO - 10.1038/s41467-017-01104-3
ID - Al Ouahabi2017
ER -

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@article{doi:10.1021/jacs.5b02639,
author = {Al Ouahabi, Abdelaziz and Charles, Laurence and Lutz, Jean-Fran{\c{c}}ois},
title = {Synthesis of Non-Natural Sequence-Encoded Polymers Using Phosphoramidite Chemistry},
journal = {Journal of the American Chemical Society},
volume = {137},
number = {16},
pages = {5629-5635},
year = {2015},
doi = {10.1021/jacs.5b02639},
note ={PMID: 25851514},
URL = {
https://doi.org/10.1021/jacs.5b02639
},
eprint = {
https://doi.org/10.1021/jacs.5b02639
}
}

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TY - JOUR
T1 - Coding Macromolecules: Inputting Information in Polymers Using Monomer-Based Alphabets
AU - Lutz, Jean-François
Y1 - 2015/07/28
PY - 2015
DA - 2015/07/28
N1 - doi: 10.1021/acs.macromol.5b00890
DO - 10.1021/acs.macromol.5b00890
T2 - Macromolecules
JF - Macromolecules
JO - Macromolecules
SP - 4759
EP - 4767
VL - 48
IS - 14
PB - American Chemical Society
SN - 0024-9297
M3 - doi: 10.1021/acs.macromol.5b00890
UR - https://doi.org/10.1021/acs.macromol.5b00890
ER -

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@article{doi:10.1137/140962486,
author = {Acharya, Jayadev and Das, Hirakendu and Milenkovic, Olgica and Orlitsky, Alon and Pan, Shengjun},
title = {String Reconstruction from Substring Compositions},
journal = {SIAM Journal on Discrete Mathematics},
volume = {29},
number = {3},
pages = {1340-1371},
year = {2015},
doi = {10.1137/140962486},
URL = {
https://doi.org/10.1137/140962486
},
eprint = {
https://doi.org/10.1137/140962486
}
,
abstract = { Motivated by mass-spectrometry protein sequencing, we consider the problem of reconstructing a string from the multisets of its substring composition. We show that all strings of length 7, one less than a prime and one less than twice a prime, can be reconstructed uniquely up to reversal. For all other lengths, we show that unique reconstruction is not always possible and provide sometimes-tight bounds on the largest number of strings with given substring compositions. The lower bounds are derived by combinatorial arguments, while the upper bounds follow from algebraic approaches that lead to precise characterizations of the sets of strings with the same substring compositions in terms of the factorization properties of bivariate polynomials. Using results on the transience of multidimensional random walks, we also provide a reconstruction algorithm that recovers random strings over alphabets of size \$\ge4\$ from their substring compositions in optimal near-quadratic time. The problem considered is related to the well-known turnpike problem, and its solution may hence shed light on this longstanding open problem as well. }
}